Clustering cancer gene expression data at the same time? - cluster-analysis

I'm working at gene expression data clustering techniques and I have downloaded 35 datasets from web,
We have 35 datasets that each of them represents a type of cancer. Each dataset has its own features. Some of these datasets are shared in several features, and some of them do not share anything from the viewpoint of features.
My question is, how do we ultimately cluster these data, while many of them do not have the same characteristics?
I think that we do the clustering operation on all 35 datasets at the same time.
Is my idea correct?
any help is appreciated.

I assume that when you say heterogenous it'll be things like different gene expression platforms where different genes are present.
You can use any clustering technique, but you'll need to write your own distance metric that takes into account heterogeneity within your dataset. For instance, you could use the correlation of all the genes that are in common between pairwise samples, create a distance matrix from this, then use something like hierarchical clustering on this distance matrix

I think there is no need to write your own distance metric. There already exists plenty of distance metrics that can work for mixed data types. For instance, the gower distance works well for mixed data type. See this post on the same. But if your data contains only continuous values then you can use k-means. You'll also be better off, if the data is preprocessed first.

Related

Best way to validate DBSCAN Clusters

I have used the ELKI implementation of DBSCAN to identify fire hot spot clusters from a fire data set and the results look quite good. The data set is spatial and the clusters are based on latitude, longitude. Basically, the DBSCAN parameters identify hot spot regions where there is a high concentration of fire points (defined by density). These are the fire hot spot regions.
My question is, after experimenting with several different parameters and finding a pair that gives a reasonable clustering result, how does one validate the clusters?
Is there a suitable formal validation method for my use case? Or is this subjective depending on the application domain?
ELKI contains a number of evaluation functions for clusterings.
Use the -evaluator parameter to enable them, from the evaluation.clustering.internal package.
Some of them will not automatically run because they have quadratic runtime cost - probably more than your clustering algorithm.
I do not trust these measures. They are designed for particular clustering algorithms; and are mostly useful for deciding the k parameter of k-means; not much more than that. If you blindly go by these measures, you end up with useless results most of the time. Also, these measures do not work with noise, with either of the strategies we tried.
The cheapest are the label-based evaluators. These will automatically run, but apparently your data does not have labels (or they are numeric, in which case you need to set the -parser.labelindex parameter accordingly). Personally, I prefer the Adjusted Rand Index to compare the similarity of two clusterings. All of these indexes are sensitive to noise so they don't work too well with DBSCAN, unless your reference has the same concept of noise as DBSCAN.
If you can afford it, a "subjective" evaluation is always best.
You want to solve a problem, not a number. That is the whole point of "data science", being problem oriented and solving the problem, not obsessed with minimizing some random quality number. If the results don't work in reality, you failed.
There are different methods to validate a DBSCAN clustering output. Generally we can distinguish between internal and external indices, depending if you have labeled data available or not. For DBSCAN there is a great internal validation indice called DBCV.
External Indices:
If you have some labeled data, external indices are great and can demonstrate how well the cluster did vs. the labeled data. One example indice is the RAND indice.https://en.wikipedia.org/wiki/Rand_index
Internal Indices:
If you don't have labeled data, then internal indices can be used to give the clustering result a score. In general the indices calculate the distance of points within the cluster and to other clusters and try to give you a score based on the compactness (how close are the points to each other in a cluster?) and
separability (how much distance is between the clusters?).
For DBSCAN, there is one great internal validation indice called DBCV by Moulavi et al. Paper is available here: https://epubs.siam.org/doi/pdf/10.1137/1.9781611973440.96
Python package: https://github.com/christopherjenness/DBCV

MANOVA - huge matrices

First, sorry by the tag as "ANOVA", it is about MANOVA (yet to become a tag...)
From the tutorials I found, all the examples use small matrices, following them would not be feasible for the case of big ones as it is the case of many studies.
I got 2 matrices for my 14 sampling points, 1 for the organisms IDs (4493 IDs) and other to chemical profile (190 variables).
The 2 matrices were correlated by spearman and based on the correlation, split in 4 clusters (k-means regarding the square euclidian clustering values), the IDs on the row and chemical profile on line.
The differences among them are somewhat clear, but to have it in a more robust way I want to perform MANOVA to show the differences between and within the clusters - that is a key factor for the conclusion, of course.
Problem is that, after 8h trying, could not even input the data in a format acceptable to the analysis.
The tutorials I found are designed to very few variables and even when I think I overcame that, the program says that my matrices can't be compared by their difference in length.
Each cluster has its own set of IDs sharing all same set of variables.
What should I do?
Thanks in advance.
Diogo Ogawa
If you have missing values in your data (which practically all data sets seem to contain) you can either remove those observations or you can create a model using those observations. Use the first approach if something about your methodology gives you conviction that there is something different about those observations. Most of the time, it is better to run the model using the missing values. In this case, use the general linear model instead of a balanced ANOVA model. The balanced model will struggle with those missing data.

KNN classification with categorical data

I'm busy working on a project involving k-nearest neighbor (KNN) classification. I have mixed numerical and categorical fields. The categorical values are ordinal (e.g. bank name, account type). Numerical types are, for e.g. salary and age. There are also some binary types (e.g., male, female).
How do I go about incorporating categorical values into the KNN analysis?
As far as I'm aware, one cannot simply map each categorical field to number keys (e.g. bank 1 = 1; bank 2 = 2, etc.), so I need a better approach for using the categorical fields. I have heard that one can use binary numbers. Is this a feasible method?
You need to find a distance function that works for your data. The use of binary indicator variables solves this problem implicitly. This has the benefit of allowing you to continue your probably matrix based implementation with this kind of data, but a much simpler way - and appropriate for most distance based methods - is to just use a modified distance function.
There is an infinite number of such combinations. You need to experiment which works best for you. Essentially, you might want to use some classic metric on the numeric values (usually with normalization applied; but it may make sense to also move this normalization into the distance function), plus a distance on the other attributes, scaled appropriately.
In most real application domains of distance based algorithms, this is the most difficult part, optimizing your domain specific distance function. You can see this as part of preprocessing: defining similarity.
There is much more than just Euclidean distance. There are various set theoretic measures which may be much more appropriate in your case. For example, Tanimoto coefficient, Jaccard similarity, Dice's coefficient and so on. Cosine might be an option, too.
There are whole conferences dedicated to the topics of similarity search - nobody claimed this is trivial in anything but Euclidean vector spaces (and actually, not even there): http://www.sisap.org/2012
The most straight forward way to convert categorical data into numeric is by using indicator vectors. See the reference I posted at my previous comment.
Can we use Locality Sensitive Hashing (LSH) + edit distance and assume that every bin represents a different category? I understand that categorical data does not show any order and the bins in LSH are arranged according to a hash function. Finding the hash function that gives a meaningful number of bins sounds to me like learning a metric space.

Matlab: K-means clustering with predefined populations

I am trying to differentiate two populations. Each population is an NxM matrix in which N is fixed between the two and M is variable in length (N=column specific attributes of each run, M=run number). I have looked at PCA and K-means for differentiating the two, but I was curious of the best practice.
To my knowledge, in K-means, there is no initial 'calibration' in which the clusters are chosen such that known bimodal populations can be differentiated. It simply minimizes the distance and assigns the data to an arbitrary number of populations. I would like to tell the clustering algorithm that I want the best fit in which the two populations are separated. I can then use the fit I get from the initial clustering on future datasets. Any help, example code, or reading material would be appreciated.
-R
K-means and PCA are typically used in unsupervised learning problems, i.e. problems where you have a single batch of data and want to find some easier way to describe it. In principle, you could run K-means (with K=2) on your data, and then evaluate the degree to which your two classes of data match up with the data clusters found by this algorithm (note: you may want multiple starts).
It sounds to like you have a supervised learning problem: you have a training data set which has already been partitioned into two classes. In this case k-nearest neighbors (as mentioned by #amas) is probably the approach most like k-means; however Support Vector Machines can also be an attractive approach.
I frequently refer to The Elements of Statistical Learning: Data Mining, Inference, and Prediction, Second Edition (Springer Series in Statistics) by Trevor Hastie (Author), Robert Tibshirani (Author), Jerome Friedman (Author).
It really depends on the data. But just to let you know K-means does get stuck at local minima so if you wanna use it try running it from different random starting points. PCA's might also be useful how ever like any other spectral clustering method you have much less control over the clustering procedure. I recommend that you cluster the data using k-means with multiple random starting points and c how it works then you can predict and learn for each the new samples with K-NN (I don't know if it is useful for your case).
Check Lazy learners and K-NN for prediction.

Clustering: a training dataset of variable data dimensions

I have a dataset of n data, where each data is represented by a set of extracted features. Generally, the clustering algorithms need that all input data have the same dimensions (the same number of features), that is, the input data X is a n*d matrix of n data points each of which has d features.
In my case, I've previously extracted some features from my data but the number of extracted features for each data is most likely to be different (I mean, I have a dataset X where data points have not the same number of features).
Is there any way to adapt them, in order to cluster them using some common clustering algorithms requiring data to be of the same dimensions.
Thanks
Sounds like the problem you have is that it's a 'sparse' data set. There are generally two options.
Reduce the dimensionality of the input data set using multi-dimensional scaling techniques. For example Sparse SVD (e.g. Lanczos algorithm) or sparse PCA. Then apply traditional clustering on the dense lower dimensional outputs.
Directly apply a sparse clustering algorithm, such as sparse k-mean. Note you can probably find a PDF of this paper if you look hard enough online (try scholar.google.com).
[Updated after problem clarification]
In the problem, a handwritten word is analyzed visually for connected components (lines). For each component, a fixed number of multi-dimensional features is extracted. We need to cluster the words, each of which may have one or more connected components.
Suggested solution:
Classify the connected components first, into 1000(*) unique component classifications. Then classify the words against the classified components they contain (a sparse problem described above).
*Note, the exact number of component classifications you choose doesn't really matter as long as it's high enough as the MDS analysis will reduce them to the essential 'orthogonal' classifications.
There are also clustering algorithms such as DBSCAN that in fact do not care about your data. All this algorithm needs is a distance function. So if you can specify a distance function for your features, then you can use DBSCAN (or OPTICS, which is an extension of DBSCAN, that doesn't need the epsilon parameter).
So the key question here is how you want to compare your features. This doesn't have much to do with clustering, and is highly domain dependant. If your features are e.g. word occurrences, Cosine distance is a good choice (using 0s for non-present features). But if you e.g. have a set of SIFT keypoints extracted from a picture, there is no obvious way to relate the different features with each other efficiently, as there is no order to the features (so one could compare the first keypoint with the first keypoint etc.) A possible approach here is to derive another - uniform - set of features. Typically, bag of words features are used for such a situation. For images, this is also known as visual words. Essentially, you first cluster the sub-features to obtain a limited vocabulary. Then you can assign each of the original objects a "text" composed of these "words" and use a distance function such as cosine distance on them.
I see two options here:
Restrict yourself to those features for which all your data-points have a value.
See if you can generate sensible default values for missing features.
However, if possible, you should probably resample all your data-points, so that they all have values for all features.