Using perl hash for comparing columns of 2 files - perl

I have asked this question(sorry for asking again, this time it is different and difficult) but I have tried a lot but did not achieve the results.
I have 2 big files (tab delimited).
first file ->
Col1 Col2 Col3 Col4 Col5 Col6 Col7 Col8
101_#2 1 H F0 263 278 2 1.5
102_#1 1 6 F1 766 781 1 1.0
103_#1 2 15 V1 526 581 1 0.0
103_#1 2 9 V2 124 134 1 1.3
104_#1 1 12 V3 137 172 1 1.0
105_#1 1 17 F2 766 771 1 1.0
second file ->
Col1 Col2 Col3 Col4
97486 H 262 279
67486 9 118 119
87486 9 183 185
248233 9 124 134
If col3 value/character (of file1) and col2 value/character (of file 2) are same and then compare col5 and col6 of file 1(like a range value) with col3 and col4 of file2, if range of file 1 is present in file 2 then return that row (from file1) and also add the extra column1 from file2 in output.
Expected output ->
Col1 Col2 Col3 Col4 Col5 Col6 Col7 Col8 Col9
101_#2 1 H F0 263 278 2 1.5 97486
103_#1 2 9 V2 124 134 1 1.3 248233
So far I have tried something with hashes->
#ARGV or die "No input file specified";
open my $first, '<',$ARGV[0] or die "Unable to open input file: $!";
open my $second,'<', $ARGV[1] or die "Unable to open input file: $!";
print scalar (<$first>);
while(<$second>){
chomp;
#line=split /\s+/;
$hash{$line[2]}=$line[3];
}
while (<$first>) {
#cols = split /\s+/;
$p1 = $cols[4];
$p2 = $cols[5];
foreach $key (sort keys %hash){
if ($p1>= "$key"){
if ($p2<=$hash{$key})
{
print join("\t",#cols),"\n";
}
}
else{ next; }
}
}
But there is no comparison of col3 value/character (of file1) and col2 value/character (of file 2)in above code.
But this is also taking lot of time and memory.Can anybody suggest how I can make it fast using hashes or hashes of hashes.Thanks a lot.
Hello everyone,
Thanks a lot for your help. I figured out an efficient way for my own question.
#ARGV or die "No input file specified";
open $first, '<',$ARGV[0] or die "Unable to open input file: $!";
open $second,'<', $ARGV[1] or die "Unable to open input file: $!";
print scalar (<$first>);
while(<$second>){
chomp;
#line=split /\s+/;
$hash{$line[1]}{$line[2]}{$line[3]}= $line[0];
}
while (<$first>) {
#cols = split /\s+/;
foreach $key1 (sort keys %hash) {
foreach $key2 (sort keys %{$hash{$key1}}) {
foreach $key3 (sort keys %{$hash{$key1}{$key2}}) {
if (($cols[2] eq $key1) && ($cols[4]>=$key2) && ($cols[5]<=$key3)){
print join("\t",#cols),"\t",$hash{$key1}{$key2}{$key3},"\n";
}
last;
}
}
}
}
Is it right?

You don't need two hash tables. You just need one hash table built from entries in the first file, and when you loop through the second file, check if there's a key in the first-file hash table using defined.
If there is a key, do your comparisons on the values of other columns (we store values from the first file in the hash table for the third column's key).
If there's no key, then either warn, die, or have the script just keep going without saying anything, if that's what you want:
#!/usr/bin/perl -w
use strict;
use warnings;
my $firstHashRef;
open FIRST, "< $firstFile" or die "could not open first file...\n";
while (<FIRST>) {
chomp $_;
my #elements = split "\t", $_;
my $col3Val = $elements[2]; # Perl arrays are zero-indexed
my $col5Val = $elements[4];
my $col6Val = $elements[5];
# keep the fifth and sixth column values on hand, for
# when we loop through the second file...
if (! defined $firstHashRef->{$col3Val}) {
$firstHashRef->{$col3Val}->{Col5} = $col5Val;
$firstHashRef->{$col3Val}->{Col6} = $col6Val;
}
}
close FIRST;
open SECOND, "< $secondFile" or die "could not open second file...\n";
while (<SECOND>) {
chomp $_;
my #elements = split "\t", $_;
my $col2ValFromSecondFile = $elements[1];
my $col3ValFromSecondFile = $elements[2];
my $col4ValFromSecondFile = $elements[3];
if (defined $firstHashRef->{$col2ValFromSecondFile}) {
# we found a matching key
# 1. Compare $firstHashRef->{$col2ValFromSecondFile}->{Col5} with $col3ValFromSecondFile
# 2. Compare $firstHashRef->{$col2ValFromSecondFile}->{Col6} with $col4ValFromSecondFile
# 3. Do something interesting, based on comparison results... (this is left to you to fill in)
}
else {
warn "We did not locate entry in hash table for second file's Col2 value...\n";
}
}
close SECOND;

How about using just awk for this -
awk '
NR==FNR && NR>1{a[$3]=$0;b[$3]=$5;c[$3]=$6;next}
($2 in a) && ($3<=b[$2] && $4>=c[$2]) {print a[$2],$1}' file1 file2
Input Data:
[jaypal:~/Temp] cat file1
Col1 Col2 Col3 Col4 Col5 Col6 Col7 Col8
101_#2 1 H F0 263 278 2 1.5
109_#2 1 H F0 263 278 2 1.5
102_#1 1 6 F1 766 781 1 1.0
103_#1 2 15 V1 526 581 1 0.0
103_#1 2 9 V2 124 134 1 1.3
104_#1 1 12 V3 137 172 1 1.0
105_#1 1 17 F2 766 771 1 1.0
[jaypal:~/Temp] cat file2
Col1 Col2 Col3 Col4
97486 H 262 279
67486 9 118 119
87486 9 183 185
248233 9 124 134
Test:
[jaypal:~/Temp] awk '
NR==FNR && NR>1{a[$3]=$0;b[$3]=$5;c[$3]=$6;next}
($2 in a) && ($3<=b[$2] && $4>=c[$2]) {print a[$2],$1}' file1 file2
101_#2 1 H F0 263 278 2 1.5 97486
103_#1 2 9 V2 124 134 1 1.3 248233

Related

How to resolve this warning in Perl

I asked this type of ques previously but didn't provide the full code.
I am reading below file and checking the max word width present in each column and then write it to another file with proper alignment.
id0 id1 id2 batch
0 34 56 70
2 3647 58 72 566
4 39 616 75 98 78 78987 9876 7899 776
89 40 62 76
8 42 64 78
34 455 544 565
My code:
unlink "temp1.log";
use warnings;
use strict;
use feature 'say';
my $log1_file = "log1.log";
my $temp1 = "temp1.log";
open(IN1, "<$log1_file" ) or die "Could not open file $log1_file: $!";
my #col_lens;
while (my $line = <IN1>) {
my #fs = split " ", $line;
my #rows = #fs ;
#col_lens = map (length, #rows) if $.==1;
for my $col_idx (0..$#rows) {
my $col_len = length $rows[$col_idx];
if ($col_lens[$col_idx] < $col_len) {
$col_lens[$col_idx] = $col_len;
}
};
};
close IN1;
open(IN1, "<$log1_file" ) or die "Could not open file $log1_file: $!";
open(tempp1,"+>>$temp1") or die "Could not open file $temp1: $!";
while (my $line = <IN1>) {
my #fs = split " ", $line;
my #az;
for my $h (0..$#fs) {
my $len = length $fs[$h];
my $blk_len = $col_lens[$h]+1;
my $right = $blk_len - $len;
$az[$h] = (" ") . $fs[$h] . ( " " x $right );
}
say tempp1 (join "|",#az);
};
My warning
Use of uninitialized value in numeric lt (<) at new.pl line 25, <IN1> line 3.
Use of uninitialized value in numeric lt (<) at new.pl line 25, <IN1> line 4.
Use of uninitialized value in numeric lt (<) at new.pl line 25, <IN1> line 4.
Use of uninitialized value in numeric lt (<) at new.pl line 25, <IN1> line 4.
Use of uninitialized value in numeric lt (<) at new.pl line 25, <IN1> line 4.
Use of uninitialized value in numeric lt (<) at new.pl line 25, <IN1> line 4.
I am getting the output correctly but don't know how to remove this warnings.
$col_idx can be up to the number of fields on a line, minus one. For the third line, this is more than the highest index of #col_lens, which contains at most 3 elements. So doing the following makes no sense:
if ($col_lens[$col_idx] < $col_len) {
$col_lens[$col_idx] = $col_len;
}
Replace it with
if (!defined($col_lens[$col_idx]) || $col_lens[$col_idx] < $col_len) {
$col_lens[$col_idx] = $col_len;
}
With this, there's really no point checking for $. == 1 anymore.
You're getting uninitialized warning because, while checking the $col_lens[$col_idx] < $col_len condition, one or both of them are undef.
Solution 1:
You can skip checking this condition by the use of next statement.
for my $col_idx (0..$#rows) {
my $col_len = length $rows[$col_idx];
next unless $col_lens[$col_idx];
if ($col_lens[$col_idx] < $col_len) {
$col_lens[$col_idx] = $col_len;
}
}
Solution 2: (Not advised):
You can simply ignore Use of uninitialized value.. warnings by putting this line at top of your script. This will disable uninitialized warnings in a block.
no warnings 'uninitialized';
For more info, please refer this link
Following code demonstrates one of many possible ways for solution to this task
read line by line
get length of each field
compare with stored earlier
adjust to max length
form $format string for print
print formatted data
use strict;
use warnings;
use feature 'say';
my(#data,#length,$format);
while ( <DATA> ) {
my #e = split ' ';
my #l = map{ length } #e;
$length[$_] = ($length[$_] // 0) < $l[$_] ? $l[$_] : $length[$_] for 0..$#e;
push #data,\#e;
}
$format = join ' ', map{ '%'.$_.'s' } #length;
$format .= "\n";
for my $row ( #data ) {
printf $format, map { $row->[$_] // '' } 0..$#length;;
}
__DATA__
id0 id1 id2 batch
0 34 56 70
2 3647 58 72 566
4 39 616 75 98 78 78987 9876 7899 776
89 40 62 76
8 42 64 78
34 455 544 565
Output
id0 id1 id2 batch
0 34 56 70
2 3647 58 72 566
4 39 616 75 98 78 78987 9876 7899 776
89 40 62 76
8 42 64 78
34 455 544 565

Modifying perl script to not print duplicates and extract sequences of a certain length

I want to first apologize for the biological nature of this post. I thought I should post some background first. I have a set of gene files that contain anywhere from one to five DNA sequences from different species. I used a bash shell script to perform blastn with each gene file as a query and a file of all transcriptome sequences (all_transcriptome_seq.fasta) from the five species as the subject. I now want to process these output files (and there are many) so that I can get all subject sequences that hit into one file per gene, with duplicate sequences removed (except to keep one), and ensure I'm getting the length of the sequences that actually hit the query.
Here is what the blastn output looks like for one gene file (columns: qseqid qlen sseqid slen qframe qstart qend sframe sstart send evalue bitscore pident nident length)
Acur_01000750.1_OFAS014956-RA-EXON04 248 Apil_comp17195_c0_seq1 1184 1 1 248 1 824 1072 2e-73 259 85.60 214 250
Acur_01000750.1_OFAS014956-RA-EXON04 248 Atri_comp5613_c0_seq1 1067 1 2 248 1 344 96 8e-97 337 91.16 227 249
Acur_01000750.1_OFAS014956-RA-EXON04 248 Acur_01000750.1 992 1 1 248 1 655 902 1e-133 459 100.00 248 248
Acur_01000750.1_OFAS014956-RA-EXON04 248 Btri_comp17734_c0_seq1 1001 1 1 248 1 656 905 5e-69 244 84.40 211 250
Btri_comp17734_c0_seq1_OFAS014956-RA-EXON04 250 Atri_comp5613_c0_seq1 1067 1 2 250 1 344 96 1e-60 217 82.33 205 249
Btri_comp17734_c0_seq1_OFAS014956-RA-EXON04 250 Acur_01000750.1 992 1 1 250 1 655 902 5e-69 244 84.40 211 250
Btri_comp17734_c0_seq1_OFAS014956-RA-EXON04 250 Btri_comp17734_c0_seq1 1001 1 1 250 1 656 905 1e-134 462 100.00 250 250
I've been working on a perl script that would, in short, take the sseqid column to pull out the corresponding sequences from the all_transcriptome_seq.fasta file, place these into a new file, and trim the transcripts to the sstart and send positions. Here is the script, so far:
#!/usr/bin/env perl
use warnings;
use strict;
use Data::Dumper;
############################################################################
# blastn_post-processing.pl v. 1.0 by Michael F., XXXXXX
############################################################################
my($progname) = $0;
############################################################################
# Initialize variables
############################################################################
my($jter);
my($com);
my($t1);
if ( #ARGV != 2 ) {
print "Usage:\n \$ $progname <infile> <transcriptomes>\n";
print " infile = tab-delimited blastn text file\n";
print " transcriptomes = fasta file of all transcriptomes\n";
print "exiting...\n";
exit;
}
my($infile)=$ARGV[0];
my($transcriptomes)=$ARGV[1];
############################################################################
# Read the input file
############################################################################
print "Reading the input file... ";
open (my $INF, $infile) or die "Unable to open file";
my #data = <$INF>;
print #data;
close($INF) or die "Could not close file $infile.\n";
my($nlines) = $#data + 1;
my($inlines) = $nlines - 1;
print "$nlines blastn hits read\n\n";
############################################################################
# Extract hits and place sequences into new file
############################################################################
my #temparray;
my #templine;
my($seqfname);
open ($INF, $infile) or die "Could not open file $infile for input.\n";
#temparray = <$INF>;
close($INF) or die "Could not close file $infile.\n";
$t1 = $#temparray + 1;
print "$infile\t$t1\n";
$seqfname = "$infile" . ".fasta";
if ( -e $seqfname ) {
print " --> $seqfname exists. overwriting\n";
unlink($seqfname);
}
# iterate through the individual hits
for ($jter=0; $jter<$t1; $jter++) {
(#templine) = split(/\s+/, $temparray[$jter]);
$com = "./extract_from_genome2 $transcriptomes $templine[2] $templine[8] $templine[9] $templine[2]";
# print "$com\n";
system("$com");
system("cat temp.3 >> $seqfname");
} # end for ($jter=0; $jter<$t1...
# Arguments for "extract_from_genome2"
# // argv[1] = name of genome file
# // argv[2] = gi number for contig
# // argv[3] = start of subsequence
# // argv[4] = end of subsequence
# // argv[5] = name of output sequence
Using this script, here is the output I'm getting:
>Apil_comp17195_c0_seq1
GATTCTTGCATCTGCAGTAAGACCAGAAATGCTCATTCCTATATGGCTATCTAATGGTATTATTTTTTTCTGATGTGCTGATAATTCAGACGAAGCTCTTTTAAGAGCCACAAGAACTGCATACTGCTTGTTTTTTACTCCAACAGTAGCAGCTCCCAGTTTTACAGCTTCCATTGCATATTCGACTTGGTGCAGGCGTCCCTGGGGACTCCAGACGGTAACGTCAGAATCATACTGGTTACGGAACA
>Atri_comp5613_c0_seq1
GAGAATTCTAGCATCAGCAGTGAGGCCTGAAATACTCATGCCTATGTGACTATCTAGAGGTATTATTTTTTTTTGATGAGCTGACAGTTCAGAAGAAGCTCTTTTGAGAGCTACAAGAACTGCATACTGTTTATTTTTTACTCCAACTGTTGCTGCTCCAAGCTTTACAGCCTCCATTGCATATTCCACTTGGTGTAAACGCCCCTGAGGACTCCATACCGTAACATCAGAATCATACTGATTACGGA
>Acur_01000750.1
GAATTCTAGCGTCAGCAGTGAGTCCTGAAATACTCATCCCTATGTGGCTATCTAGAGGTATTATTTTTTCTGATGGGCCGACAGTTCAGAGGATGCTCTTTTAAGAGCCACAAGAACTGCATACTCTTTATTTTTACTCCAACAGTAGCAGCTCCAAGCTTCACAGCCTCCATTGCATATTCCACCTGGTGTAAACGTCCCTGAGGGCTCCATACCGTAACATCAGAATCATACTGGTTACGGAACA
>Btri_comp17734_c0_seq1
GAATCCTTGCATCTGCAGTAAGTCCAGAAATGCTCATTCCAATATGGCTATCTAATGGTATTATTTTTTTCTGGTGAGCAGACAATTCAGATGATGCTCTTTTAAGAGCTACCAGTACTGCAAAATCATTGTTCTTCACTCCAACAGTTGCAGCACCTAATTTGACTGCCTCCATTGCATACTCCACTTGGTGCAATCTTCCCTGAGGGCTCCATACCGTAACATCAGAATCATACTGGTTACGGAACA
>Atri_comp5613_c0_seq1
GAGAATTCTAGCATCAGCAGTGAGGCCTGAAATACTCATGCCTATGTGACTATCTAGAGGTATTATTTTTTTTTGATGAGCTGACAGTTCAGAAGAAGCTCTTTTGAGAGCTACAAGAACTGCATACTGTTTATTTTTTACTCCAACTGTTGCTGCTCCAAGCTTTACAGCCTCCATTGCATATTCCACTTGGTGTAAACGCCCCTGAGGACTCCATACCGTAACATCAGAATCATACTGATTACGGA
>Acur_01000750.1
GAATTCTAGCGTCAGCAGTGAGTCCTGAAATACTCATCCCTATGTGGCTATCTAGAGGTATTATTTTTTCTGATGGGCCGACAGTTCAGAGGATGCTCTTTTAAGAGCCACAAGAACTGCATACTCTTTATTTTTACTCCAACAGTAGCAGCTCCAAGCTTCACAGCCTCCATTGCATATTCCACCTGGTGTAAACGTCCCTGAGGGCTCCATACCGTAACATCAGAATCATACTGGTTACGGAACA
>Btri_comp17734_c0_seq1
GAATCCTTGCATCTGCAGTAAGTCCAGAAATGCTCATTCCAATATGGCTATCTAATGGTATTATTTTTTTCTGGTGAGCAGACAATTCAGATGATGCTCTTTTAAGAGCTACCAGTACTGCAAAATCATTGTTCTTCACTCCAACAGTTGCAGCACCTAATTTGACTGCCTCCATTGCATACTCCACTTGGTGCAATCTTCCCTGAGGGCTCCATACCGTAACATCAGAATCATACTGGTTACGGAACA
As you can see, it's pretty close to what I'm wanting. Here are the two issues I have and cannot seem to figure out how to resolve with my script. The first is that a sequence may occur more than once in the sseqid column, and with the script in its current form, it will print out duplicates of these sequences. I only need one. How can I modify my script to not duplicate sequences (i.e., how do I only retain one but remove the other duplicates)? Expected output:
>Apil_comp17195_c0_seq1
GATTCTTGCATCTGCAGTAAGACCAGAAATGCTCATTCCTATATGGCTATCTAATGGTATTATTTTTTTCTGATGTGCTGATAATTCAGACGAAGCTCTTTTAAGAGCCACAAGAACTGCATACTGCTTGTTTTTTACTCCAACAGTAGCAGCTCCCAGTTTTACAGCTTCCATTGCATATTCGACTTGGTGCAGGCGTCCCTGGGGACTCCAGACGGTAACGTCAGAATCATACTGGTTACGGAACA
>Atri_comp5613_c0_seq1
GAGAATTCTAGCATCAGCAGTGAGGCCTGAAATACTCATGCCTATGTGACTATCTAGAGGTATTATTTTTTTTTGATGAGCTGACAGTTCAGAAGAAGCTCTTTTGAGAGCTACAAGAACTGCATACTGTTTATTTTTTACTCCAACTGTTGCTGCTCCAAGCTTTACAGCCTCCATTGCATATTCCACTTGGTGTAAACGCCCCTGAGGACTCCATACCGTAACATCAGAATCATACTGATTACGGA
>Acur_01000750.1
GAATTCTAGCGTCAGCAGTGAGTCCTGAAATACTCATCCCTATGTGGCTATCTAGAGGTATTATTTTTTCTGATGGGCCGACAGTTCAGAGGATGCTCTTTTAAGAGCCACAAGAACTGCATACTCTTTATTTTTACTCCAACAGTAGCAGCTCCAAGCTTCACAGCCTCCATTGCATATTCCACCTGGTGTAAACGTCCCTGAGGGCTCCATACCGTAACATCAGAATCATACTGGTTACGGAACA
>Btri_comp17734_c0_seq1
GAATCCTTGCATCTGCAGTAAGTCCAGAAATGCTCATTCCAATATGGCTATCTAATGGTATTATTTTTTTCTGGTGAGCAGACAATTCAGATGATGCTCTTTTAAGAGCTACCAGTACTGCAAAATCATTGTTCTTCACTCCAACAGTTGCAGCACCTAATTTGACTGCCTCCATTGCATACTCCACTTGGTGCAATCTTCCCTGAGGGCTCCATACCGTAACATCAGAATCATACTGGTTACGGAACA
The second is the script is not quite extracting the right base pairs. It's super close, off by one or two, but its not exact.
For example, take the first subject hit Apil_comp17195_c0_seq1. The sstart and send values are 824 and 1072, respectively. When I go to the all_transcriptome_seq.fasta, I get
AAGATTCTTGCATCTGCAGTAAGACCAGAAATGCTCATTCCTATATGGCTATCTAATGGTATTATTTTTTTCTGATGTGCTGATAATTCAGACGAAGCTCTTTTAAGAGCCACAAGAACTGCATACTGCTTGTTTTTTACTCCAACAGTAGCAGCTCCCAGTTTTACAGCTTCCATTGCATATTCGACTTGGTGCAGGCGTCCCTGGGGACTCCAGACGGTAACGTCAGAATCATACTGGTTACGGAAC
at that base pair range, not
GATTCTTGCATCTGCAGTAAGACCAGAAATGCTCATTCCTATATGGCTATCTAATGGTATTATTTTTTTCTGATGTGCTGATAATTCAGACGAAGCTCTTTTAAGAGCCACAAGAACTGCATACTGCTTGTTTTTTACTCCAACAGTAGCAGCTCCCAGTTTTACAGCTTCCATTGCATATTCGACTTGGTGCAGGCGTCCCTGGGGACTCCAGACGGTAACGTCAGAATCATACTGGTTACGGAACA
as outputted by my script, which is what I'm expecting. You will also notice that the sequence outputted by my script is slightly shorter than it should be. Does anyone know how I can fix these issues in my script?
Thanks, and sorry for the lengthy post!
Edit 1: a solution was offered that work for some of the infiles. However, some were causing the script to output fewer sequences than expected. Here is one such infile with 9 hits, from which I was expecting only 4 sequences.
Note: this issue has been largely resolved based on the solution provided below the answer section
Apil_comp16418_c0_seq1_OFAS000119-RA-EXON01 1587 Apil_comp16418_c0_seq1 2079 1 1 1587 1 416 2002 0.0 2931 100.00 1587 1587
Apil_comp16418_c0_seq1_OFAS000119-RA-EXON01 1587 Atri_comp13712_c0_seq1 1938 1 1 1587 1 1651 75 0.0 1221 80.73 1286 1593
Apil_comp16418_c0_seq1_OFAS000119-RA-EXON01 1587 Ctom_01003023.1 2162 1 1 1406 1 1403 1 0.0 1430 85.07 1197 1407
Atri_comp13712_c0_seq1_OFAS000119-RA-EXON01 1441 Apil_comp16418_c0_seq1 2079 1 1 1437 1 1866 430 0.0 1170 81.43 1175 1443
Atri_comp13712_c0_seq1_OFAS000119-RA-EXON01 1441 Atri_comp13712_c0_seq1 1938 1 1 1441 1 201 1641 0.0 2662 100.00 1441 1441
Atri_comp13712_c0_seq1_OFAS000119-RA-EXON01 1441 Acur_01000228.1 2415 1 1 1440 1 2231 797 0.0 1906 90.62 1305 1440
Ctom_01003023.1_OFAS000119-RA-EXON01 1289 Apil_comp16418_c0_seq1 2079 1 3 1284 1 1714 430 0.0 1351 85.69 1102 1286
Ctom_01003023.1_OFAS000119-RA-EXON01 1289 Acur_01000228.1 2415 1 1 1287 1 2084 797 0.0 1219 83.81 1082 1291
Ctom_01003023.1_OFAS000119-RA-EXON01 1289 Ctom_01003023.1 2162 1 1 1289 1 106 1394 0.0 2381 100.00 1289 1289
Edit 2: There is still an occasional output lacking fewer sequences than expected, although not as many after incorporating modifications to my script from Edit 1 suggestion (i.e., accounting for reverse direction). I cannot figure out why the script would be outputting fewer sequences in these other cases. Below the infile in question. The output is lacking Btri_comp15171_c0_seq1:
Apil_comp19456_c0_seq1_OFAS000248-RA-EXON07 2464 Apil_comp19456_c0_seq1 3549 1 1 2464 1 761 3224 0.0 4551 100.00 2464 2464
Apil_comp19456_c0_seq1_OFAS000248-RA-EXON07 2464 Btri_comp15171_c0_seq1 3766 1 1 2456 1 3046 591 0.0 1877 80.53 1985 2465
Btri_comp15171_c0_seq1_OFAS000248-RA-EXON07 2457 Apil_comp19456_c0_seq1 3549 1 1 2457 1 3214 758 0.0 1879 80.54 1986 2466
Btri_comp15171_c0_seq1_OFAS000248-RA-EXON07 2457 Atri_comp28646_c0_seq1 1403 1 1256 2454 1 1401 203 0.0 990 81.60 980 1201
Btri_comp15171_c0_seq1_OFAS000248-RA-EXON07 2457 Btri_comp15171_c0_seq1 3766 1 1 2457 1 593 3049 0.0 4538 100.00 2457 2457
You can use hash to remove duplicates
The bellow code remove duplicates depending on their subject length (keep larger subject length rows).
Just update your # iterate through the individual hits part with
# iterate through the individual hits
my %filterhash;
my $subject_length;
for ($jter=0; $jter<$t1; $jter++) {
(#templine) = split(/\s+/, $temparray[$jter]);
$subject_length = $templine[9] -$templine[8];
if(exists $filterhash{$templine[2]} ){
if($filterhash{$templine[2]} < $subject_length){
$filterhash{$templine[2]}= $subject_length;
}
}
else{
$filterhash{$templine[2]}= $subject_length;
}
}
my %printhash;
for ($jter=0; $jter<$t1; $jter++) {
(#templine) = split(/\s+/, $temparray[$jter]);
$subject_length = $templine[9] -$templine[8];
if(not exists $printhash{$templine[2]})
{
$printhash{$templine[2]}=1;
if(exists $filterhash{$templine[2]} and $filterhash{$templine[2]} == $subject_length ){
$com = "./extract_from_genome2 $transcriptomes $templine[2] $templine[8] $templine[9] $templine[2]";
# print "$com\n";
system("$com");
system("cat temp.3 >> $seqfname");
}
}
else{
if(exists $filterhash{$templine[2]} and $filterhash{$templine[2]} == $subject_length ){
$com = "./extract_from_genome2 $transcriptomes $templine[2] $templine[8] $templine[9] $templine[2]";
#print "$com\n";
system("$com");
system("cat temp.3 >> $seqfname");
}
}
} # end for ($jter=0; $jter<$t1...
Hope this will help you.
Edit part update
for negative stand you need to replace
$subject_length = $templine[9] -$templine[8];
with
if($templine[8] > $templine[9]){
$subject_length = $templine[8] -$templine[9];
}else{
$subject_length = $templine[9] -$templine[8];
}
You also need to update your extract_from_genome2 code for negative strand sequences.

Perl: find sum and average of specific columns

I want to calculate the average over all itemsX (where X is a digit) for each row in Perl on windows.
I have file in format:
id1 item1 cart1 id2 item2 cart2 id3 item3 cart3
0 11 34 1 22 44 2 44 44
1 44 44 55 66 34 45 55 33
Want to find sum of item blocks and their average.
Any help on this?
Here's what I've tried so far:
use strict;
use warnings;
open my $fh, '<', "files.txt" or die $!;
my $total = 0;
my $count = 0;
while (<$fh>) {
my ($item1, $item2, ) = split;
$total += $numbers;
$count += 1;
}
For the first line of input (the column names), we store the indices of the columns that start with item. For each subsequent line, we sum the columns referenced by the array slice derived from #indices.
use strict;
use warnings;
use List::Util qw(sum);
my #indices;
while (<DATA>) {
my #fields = split;
if ($. == 1) {
#indices = grep { $fields[$_] =~ /^item/ } 0 .. $#fields;
next;
}
my $sum = sum(#fields[#indices]);
my $avg = $sum / scalar(#indices);
printf("Row %d stats: sum=%d, avg=%.2f\n", $., $sum, $avg);
}
__DATA__
id1 item1 cart1 id2 item2 cart2 id3 item3 cart3
0 11 34 1 22 44 2 44 44
1 44 44 55 66 34 45 55 33
Output:
Row 2 stats: sum=77, avg=25.67
Row 3 stats: sum=165, avg=55.00

Fastest way to index and query huge tab delimited file

I have 30Gb tab-delimited text file with numbers, I need the fastest way index it and to do a query to it by first and second column. I've tried MongoDB but it takes huge time to upload data to database, I've tried mongoimport via json file but it takes huge amount of time.
mongoimport --upsert --upsertFields A,B,S1,E1,S2,E2 -d DBName -c
TableName data.json
Data file fragment:
504 246 91.92007 93 0 4657 5631 5911 0 39 1061 1162
813 469 92.14697 109 0 2057 2665 7252 1 363 961 1399
2388 987 92.20945 61 0 1183 1575 1824 0 66 560 5088
2388 2323 92.88472 129 0 75 1161 1824 1 2516 3592 12488
2729 1008 95.29058 47 0 435 1166 1193 1 76 654 1055
2757 76 94.25837 12 0 0 44 1946 0 51 68 247
2757 2089 92.63158 14 0 12 30 1946 0 14 30 211
What is the right efficient way to do it with minimum time? Any hints about the best database for it? Or about mongo upload speed optimisation?
Query examples:
objs = db.TableName.find({'A':2757})
objs = db.TableName.find({'B':76})
For each number in column A and B there are up to 1000 hits with the mean 20.
Databases often has complex work to do in order to be more robust.
If you use strait B-tree indexes, normally it is faster.
Following you'll find a upload script in perl.
#!/usr/bin/perl
use DB_File;
use Fcntl ;
# $DB_BTREE->{'cachesize'} = 1000000;
$DB_BTREE->{'flags'} = R_DUP ;
my (%h, %h1, %h2,$n);
my $x = tie %h, 'DB_File', "bf.db", O_RDWR|O_CREAT|O_TRUNC , 0640, $DB_BTREE;
my $x1= tie %h1, 'DB_File', "i1.db", O_RDWR|O_CREAT|O_TRUNC , 0640, $DB_BTREE;
my $x2= tie %h2, 'DB_File', "i2.db", O_RDWR|O_CREAT|O_TRUNC , 0640, $DB_BTREE;
while(<>){ chomp;
if(/(\d+)\s+(\d+)/){
$h{++$n}=$_; ## add the tup
$h1{$1} = $n; ## add to index1
$h2{$2} = $n ## add to index2;
}
}
untie %h;
untie %h1;
untie %h2;
and a query:
#!/usr/bin/perl
use DB_File;
use Fcntl ;
$DB_BTREE->{'flags'} = R_DUP ;
my (%h, %h1, %h2, $n, #list);
my $x = tie %h, 'DB_File', "bf.db", O_RDWR|O_CREAT , 0640, $DB_BTREE;
my $x1= tie %h1, 'DB_File', "i1.db", O_RDWR|O_CREAT , 0640, $DB_BTREE;
my $x2= tie %h2, 'DB_File', "i2.db", O_RDWR|O_CREAT , 0640, $DB_BTREE;
while(<>){ chomp; # Queries input format: A:number or B:number
if(/A:(\d+)/){
#list = sort $x1->get_dup($1) ;
for(#list){print $h{$_},"\n"; }
}
if(/B:(\d+)/){
#list = sort $x2->get_dup($1) ;
for(#list){print $h{$_},"\n"; }
}
}
Query is very fast.
But upload took 20s (user time) for 1 000 000 lines...
(please if you do experiments with your data, show us the times)

Combining duplicated lines in txt file with perl

I am trying to combine duplicate lines using Perl with little luck. My tab-delimited text file is structured as follows (spaces added for readability):
Pentamer Probability Observed Length
ATGCA 0.008 1 16
TGTAC 0.021 1 16
GGCAT 0.008 1 16
CAGTG 0.004 1 16
ATGCA 0.016 2 23
TGTAC 0.007 1 23
I would like to be combine duplicated lines by adding the three numeric columns, therefor the line containing "ATGCA" would now look like this:
ATGCA 0.024 3 39
Any ideas/help/suggestions would be greatly appreciated! Thanks!
#!/usr/bin/perl
use warnings;
use strict;
my %hash;
while(<>) {
my #v = split(/\s+/);
if (defined $hash{$v[0]}) {
my $arr = $hash{$v[0]};
$hash{$v[0]} = [$v[0], $arr->[1] + $v[1],
$arr->[2] + $v[2], $arr->[3] + $v[3]];
} else {
$hash{$v[0]} = [#v];
}
}
foreach my $key (keys %hash) {
print join(" ", #{$hash{$key}}), "\n";
}
Here's another option:
use Modern::Perl;
my %hash;
while ( my $line = <DATA> ) {
my #vals = split /\s+/, $line;
$hash{ $vals[0] }->[$_] += $vals[ $_ + 1 ] for 0 .. 2;
}
say join "\t", $_, #{ $hash{$_} } for sort keys %hash;
__DATA__
ATGCA 0.008 1 16
TGTAC 0.021 1 16
GGCAT 0.008 1 16
CAGTG 0.004 1 16
ATGCA 0.016 2 23
TGTAC 0.007 1 23
Output:
ATGCA 0.024 3 39
CAGTG 0.004 1 16
GGCAT 0.008 1 16
TGTAC 0.028 2 39