match columns on different lines and sum - perl

Question

I have a csv with about 160,000 lines, it looks like this:
chr1,160,161,3,0.333333333333333,+
chr1,161,162,4,0.5,-
chr1,309,310,14,0.0714285714285714,+
chr1,311,312,2,0.5,-
chr1,499,500,39,0.717948717948718,+
chr2,500,501,8,0.375,-
chr2,510,511,18,0.5,+
chr2,511,512,6,0.333333333333333,-
I would like to pair lines where column 1 is the same, column 3 matches column 2 and where column 6 is a '+' while on the other line it is a '-'. If this is true I would like to sum column 4 and column 5.
My desired out put would be
chr1,160,161,7,0.833333333333333,+
chr1,309,310,14,0.0714285714285714,+
chr1,311,312,2,0.5,-
chr1,499,500,39,0.717948717948718,+
chr2,500,501,8,0.375,-
chr2,510,511,24,0.833333333333333,-
the best solution I can think of is to duplicate the file and then match columns between the file and it's duplicate with perl:
#!/usr/bin/perl
use strict;
use warnings;
open my $firstfile, '<', $ARGV[0] or die "$!";
open my $secondfile, '<', $ARGV[1] or die "$!";
my ($chr_a, $chr_b,$start,$end,$begin,$finish, $sum_a, $sum_b, $total_a,
$total_b,$sign_a,$sign_b);
while (<$firstfile>) {
my #col = split /,/;
$chr_a = $col[0];
$start = $col[1];
$end = $col[2];
$sum_a = $col[3];
$total_a = $col[4];
$sign_a = $col[5];
seek($secondfile,0,0);
while (<$secondfile>) {
my #seccol = split /,/;
$chr_b = $seccol[0];
$begin = $seccol[1];
$finish = $seccol[2];
$sum_b = $seccol[3];
$total_b = $seccol[4];
$sign_b = $seccol[5];
print join ("\t", $col[0], $col[1], $col[2], $col[3]+=$seccol[3],
$col[4]+=$seccol[4], $col[5]),
"\n" if ($chr_a eq $chr_b and $end==$begin and $sign_a ne $sign_b);
}
}
And that works fine, but ideally I'd like to be able to do this within the file itself without having to duplicate it, because I have many files and so I would like to run a script over all of them that is less time-consuming.
Thanks.

Answer 1

In the absence of a response to my comment, this program will do as you ask with the data you provide.
use strict;
use warnings;
my #last;
while (<DATA>) {
s/\s+\z//;
my #line = split /,/;
if (#last
and $last[0] eq $line[0]
and $last[2] eq $line[1]
and $last[5] eq '+' and $line[5] eq '-') {
$last[3] += $line[3];
$last[4] += $line[4];
print join(',', #last), "\n";
#last = ()
}
else {
print join(',', #last), "\n" if #last;
#last = #line;
}
}
print join(',', #last), "\n" if #last;
__DATA__
chr1,160,161,3,0.333333333333333,+
chr1,161,162,4,0.5,-
chr1,309,310,14,0.0714285714285714,+
chr1,311,312,2,0.5,-
chr1,499,500,39,0.717948717948718,+
chr2,500,501,8,0.375,-
chr2,510,511,18,0.5,+
chr2,511,512,6,0.333333333333333,-
output
chr1,160,161,7,0.833333333333333,+
chr1,309,310,14,0.0714285714285714,+
chr1,311,312,2,0.5,-
chr1,499,500,39,0.717948717948718,+
chr2,500,501,8,0.375,-
chr2,510,511,24,0.833333333333333,+