Creating a stacked tiff file causes image offset - matlab

Question

I have a an image processing program whose results need to be saved to a stacked tiff file. The code for the method that does this is included below. When the resulting image is opened in ImageJ it gives the notification "Unexpected image offset" and only can display the first image in the stack.
fig = figure;
figure(fig);
fileN = handles.fileName;
[m n] = size(fileN);
m = 0;
for i=1:n
if(fileN(i) == '.')
m = i;
break
end
end
fileN = fileN(:,1:m-1);
fileP = handles.filePath;
saveFilePath = strcat(fileP,fileN,'-snake-analysis.tif')
im = imread(handles.fileFull, 1);
imshow(im, []);
hold on
plot(handles.xsFinal(1,:), handles.ysFinal(1,:), 'r-');
hold off
saveas(fig, saveFilePath);
for i=2:handles.numFrames
im = imread(handles.fileFull, i);
imshow(im, []);
hold on
plot(handles.xsFinal(i,:), handles.ysFinal(i,:), 'r-');
hold off
saveas(fig, 'inter.tif');
a = imread('inter.tif');
imwrite(a, saveFilePath,'Compression', 'none','WriteMode','append');
delete 'inter.tif';
end
Does anyone know what is causing this problem?

Answer 1

The built-in ImageJ 1.x TIFF opener does not fully handle the baseline TIFF specification: it cannot handle out-of-sequence planes.
If you use ImageJ2, you can use the SCIFIO library to open your TIFF via the File ▶ Import ▶ Image... command. The Bio-Formats Importer plugin can also open such TIFF files.