Can't locate Bio/SeqIO.pm in #INC - perl

Question

Hi I am trying to install VelvetOptimizer using docker. and it say Can't locate Bio/SeqIO.pm in #INC. It requires the following to be installed:
Velvet >= 0.7.51 ,Perl >= 5.8, BioPerl >= 1.4 and i have installed them
Please find the Dockerfile:
FROM amazonlinux
WORKDIR /shared
RUN yum -y install gcc
ADD http://www.cpan.org/src/5.0/perl-5.16.1.tar.gz /shared
RUN tar -xzf perl-5.16.1.tar.gz
WORKDIR /shared/perl-5.16.1
RUN ./Configure -des -Dprefix=/shared/perl-5.16.1/localperl
RUN make
RUN make test
RUN make install
RUN echo “Perl Installation Complete”
ENV PATH=/shared/perl-5.16.1/localperl/bin/:${PATH}
WORKDIR /shared
ADD http://www.ebi.ac.uk/~zerbino/velvet/velvet_latest.tgz .
RUN tar -xvf /shared/velvet_latest.tgz
WORKDIR /shared/velvet_1.2.10/
RUN make
ENV PATH=${PATH}:/shared/velvet_1.2.10/
WORKDIR /shared
RUN echo "BIO PERL INSTALLATION"
RUN \wget -O - https://install.perlbrew.pl | bash
ENV PATH=${PATH}:/root/perl5/perlbrew/bin/
RUN perlbrew install-cpanm
RUN cpanm Bio::Perl
WORKDIR /shared
ADD http://www.vicbioinformatics.com/VelvetOptimiser-2.2.5.tar.gz .
RUN tar zxvf VelvetOptimiser-2.2.5.tar.gz
ENV PATH=${PATH}:/shared/VelvetOptimiser-2.2.4
When I run the command/shared/VelvetOptimiser-2.2.5/VelvetOptimiser.pl
I get the below error:
Error:
Can't locate Bio/SeqIO.pm in #INC (#INC contains: /shared/VelvetOptimiser-2.2.5 /shared/vcftools/src/perl /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /shared/VelvetOptimiser-2.2.5/VelvetOpt/Assembly.pm line 238.
BEGIN failed--compilation aborted at /shared/VelvetOptimiser-2.2.5/VelvetOpt/Assembly.pm line 238.
Compilation failed in require at /shared/VelvetOptimiser-2.2.5/VelvetOptimiser.pl line 37.
BEGIN failed--compilation aborted at /shared/VelvetOptimiser-2.2.5/VelvetOptimiser.pl line 37.
But when I use "find" command: I am able to find the files.
./root/.cpanm/work/1501770319.1/BioPerl-1.007001/blib/lib/Bio/SeqIO.pm
./root/.cpanm/work/1501770319.1/BioPerl-1.007001/Bio/SeqIO.pm
./shared/perl-5.16.1/localperl/lib/site_perl/5.16.1/Bio/SeqIO.pm
I think its not be found in Perl's include path, which is represented by the variable named #INC.
Can anyone please let me know how to fix this issue. how to add it in perls include path.

Answer 1

Clearly, /shared/VelvetOptimiser-2.2.5/VelvetOptimiser.pl has
#!/usr/bin/env perl
as its shebang line. So, it will use the first perl it finds in the $PATH.
You likely have a perl in /usr/bin. In your Docker file, you have:
ENV PATH=${PATH}:/root/perl5/perlbrew/bin/
That is, you likely put /usr/bin ahead of the perlbrew directory. So, /usr/bin/env perl is picking up /usr/bin/perl and not the perl you installed via perlbrew (which is also where you installed your new perl).
So, try
ENV PATH=/root/perl5/perlbrew/bin/:${PATH}
In addition, I do not see you actually install a perl via perlbrew:
You need
perlbrew install 5.xx.x
perlbrew switch 5.xx.x
before installing cpanm and installing Bio::Perl.